advice needed can i fit a blitz car dump valve on 200tdi

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Don't forget a 100 shot of nitrous too!
With that little lot you'll need the biggest sequential blow off valve you can get.
 
But don't forget that due to the lack of differential pressure because you have no butterfly to make the turbo stall you'll need to trigger your sequential blow off valve electronically!
 
I do hope you aren't refering to me???

But I know what you mean with the other posts....pinches of salt abound, plus the odd whiff of sarcasm...!!!

No not you Ant. The OP and his mate know as much about engines as i do about intergalactical teleportation, the rest is a **** take. :):)
 
No not you Ant. The OP and his mate know as much about engines as i do about intergalactical teleportation, the rest is a **** take. :):)

Intergalactic teleportation is very simple if you have a hybrid flux capacitor! My mate makes em. That next on the list after turboing my k series freelander at least me BOV will work. Looking at 500 bhp with nitrous and urea injection:) luckily I found a supplier of kryptonite head gaskets:D
 
Intergalactic teleportation is very simple if you have a hybrid flux capacitor! My mate makes em. That next on the list after turboing my k series freelander at least me BOV will work. Looking at 500 bhp with nitrous and urea injection:) luckily I found a supplier of kryptonite head gaskets:D
Urea Injection - I heard about that....

From what I can gather from the initial research carried out recently in the Filton Wind Tunnel, the performance increases were dependant on the Ochre Rating of the Urea used.

If it was a Clear mix, the performane increases were minimal, but when they went up the Ochre scale to the stronger Yellow/Orange Urea, the performance gains were quite unprecidented.

Of course the application of the Urea through a 12 Micron Atomiser prior to the precombustion chamber was the final part of the puzzle.

According to the lead engineer, he said that extracting the Urea was the first principle obstacle they came across, but once it had been extracted and refined, the rest of the injection process was simple.
 
Urea Injection - I heard about that....

From what I can gather from the initial research carried out recently in the Filton Wind Tunnel, the performance increases were dependant on the Ochre Rating of the Urea used.

If it was a Clear mix, the performane increases were minimal, but when they went up the Ochre scale to the stronger Yellow/Orange Urea, the performance gains were quite unprecidented.

Of course the application of the Urea through a 12 Micron Atomiser prior to the precombustion chamber was the final part of the puzzle.

According to the lead engineer, he said that extracting the Urea was the first principle obstacle they came across, but once it had been extracted and refined, the rest of the injection process was simple.

Yupp it's all down to the extraction process;) I've started already!
 
Yupp it's all down to the extraction process;) I've started already!
Have you got that down to an art yet as I have a few questions if I may from an extraction meister so to speak:

So if I separate the membrane / soluble proteins using Prot-Two Kit from Sigma. My final samples are dissolved in 40 mM Tris, 7M urea, 2M thiourea and 1% C7BzO detergent, pH 10.4 (and they have already been treated with TBP/IAA). Do you think that these extracts are compatible with SDS-PAGE/Western blotting?

First, I was wondering about pH - I'm afraid if I mix those samples (pH 10.4) with my 6X sample buffer, the final pH value will hardly be 6.8, won't be? Is correct pH of sample critical for running and protein resolving?

Second, is it possible to heat those urea-based buffers as I do in 'standard' protocol to enhance protein denaturation (65C, 15 min)? In the product info, there is written that these buffers should not be heated above 30C to avoid formation of cyanates which could damage proteins.
Is the combination of urea/thiourea/detergent at above mentioned concentrations enough for effective protein denaturation (so there is no need to heat samples), or could be better denaturation achieved with heating in the presence of e.g. SDS?

If I decide to precipitate proteins (or maybe ultrafiltration could do a good job as well?), what would you suggest for protein solubilization? Just 1x sample buffer for SDS-PAGE? (I don't need to measure protein concentrations, so bromophenol blue would not interfere)

Any advice is greatly appreciated.....
 
Have you got that down to an art yet as I have a few questions if I may from an extraction meister so to speak:

So if I separate the membrane / soluble proteins using Prot-Two Kit from Sigma. My final samples are dissolved in 40 mM Tris, 7M urea, 2M thiourea and 1% C7BzO detergent, pH 10.4 (and they have already been treated with TBP/IAA). Do you think that these extracts are compatible with SDS-PAGE/Western blotting?

First, I was wondering about pH - I'm afraid if I mix those samples (pH 10.4) with my 6X sample buffer, the final pH value will hardly be 6.8, won't be? Is correct pH of sample critical for running and protein resolving?

Second, is it possible to heat those urea-based buffers as I do in 'standard' protocol to enhance protein denaturation (65C, 15 min)? In the product info, there is written that these buffers should not be heated above 30C to avoid formation of cyanates which could damage proteins.
Is the combination of urea/thiourea/detergent at above mentioned concentrations enough for effective protein denaturation (so there is no need to heat samples), or could be better denaturation achieved with heating in the presence of e.g. SDS?

If I decide to precipitate proteins (or maybe ultrafiltration could do a good job as well?), what would you suggest for protein solubilization? Just 1x sample buffer for SDS-PAGE? (I don't need to measure protein concentrations, so bromophenol blue would not interfere)

Any advice is greatly appreciated.....

I think this is getting a bit off topic now
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What I want to know, is if Blitz actually list the 200tdi in they're catalog!
 
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